DETERMINAÇÃO DO CARIÓTIPO E STATUS DO GENE BIRC5 EM CÉLULAS DE LINHAGEM DE NEUROBLASTOMA

INTRODUCTION: Neuroblastoma is the most common solid tumor in children under 1 year of age and accounts for 6% of all pediatric cancer cases. The disease caused by this tumor exhibits significant heterogeneity and disparity in prognosis due to the different forms of presentation of the tumor at diagnosis, along with epidemiological and biological factors. Among the biological factors impacting prognosis are chromosomal alterations, such as gains on chromosome 17q, which can lead to overexpression of the BIRC5 gene located at 17q25.3, which encodes the protein Survivin, a member of the inhibitor of apoptosis protein (IAP) family. AIMS: The present study aimed to select cellular models to study the impact of Survivin expression on cellular resistance to chemotherapy. MATERIALS AND METHODS: A G-banding karyotyping was used to evaluate the 17q status in eight human neuroblastoma cell lines. The cellular location of Survivin was accessed using immunofluorescence microscopy staining. RESULTS: Out of the eight cell lines, four were excluded from the study due polyploid or acrocentric chromosomes with strongly marked centromeres. The remaining cell lines showed complex and aneuploid karyograms. The immunofluorescence protocol was standardized, allowing future studies to detect the cellular localization of Survivin to be carried out by the laboratory. FINAL CONSIDERATIONS: Among the four cell lines from which karyotypes were obtained, only one showed an alteration in chromosome 17. The absence of alterations on this chromosome suggests that it must be preserved during mitosis, as it contains genes associated with tumorigenesis in Neuroblastoma. It implies that those cell lines can be used to further characterize a cellular model for the study of survivin in vitro.

KEYWORDS: Neuroblastoma; Survivin; BIRC5; Chromosome 17; Celular model.